Invitrogen insect cell manual

insect cells to generate recombinant baculovirus. • A control expression plasmid containing the Gus and or CAT gene that allows production of a recombinant baculovirus which, when used to infect insect cells, expresses the Arabidopsis thaliana β-glucuronidase and or chloramphenicol acetyl-transferase. INSECTOGENE Manual EN 3 1. General Guidelines Specifications Application Transfection of insect cells with nucleic acids Formulation Cationic lipids and colipids in water Assays Up to (6-well) or up to 75 (60 mm) per 1 ml reagent Sterility tested Cell Culture tested Storage 4°C Quality Control Standard transfection assay. The Countess automated cell counter uses state-of-the-art optics and image analysis to automate cell counting. The Countess™ automated cell counter is a benchtop counter designed to measure cell count and viability (live, dead, and total cells) accurately and precisely, using the standard trypan blue technique.

Cell Culture Cell culture is one of the major tools used in cellular and molecular biology, providing excellent model systems for studying the normal physiology and biochemistry of cells (e.g., metabolic studies, aging), the effects of drugs and toxic compounds on the cells. Isolation and transfection of bacmid DNA to Sf21 cells was carried out according to the Bac-to-Bac baculovirus expression system manual (Gibco-BRL). The bacmid-derived budded virus (BV) inocu-lum was defined as passage 1 (P1) and was used to initiate undiluted serial passaging in Sf21 cells at an initial m.o.i. of TCID 50 units per cell. Plasmid DNA was isolated and sequenced, and the construct was transfected into High Five™ insect cells using Cellfectin®-mediated transfection following the protocol described in the Invitrogen InsectSelect™ system manual (Version F, page 11) using Express Five® serum-free medium in mm culture plates Production of App1 using the.

insect cells to generate recombinant baculovirus. • A control expression plasmid containing the Gus and or CAT gene that allows production of a recombinant baculovirus which, when used to infect insect cells, expresses the Arabidopsis thaliana β-glucuronidase and or chloramphenicol acetyl-transferase. The Sf9 insect cell line is a clonal isolate derived from the parental Spodoptera frugiperda cell line IPLB-SfAE1,2, and is a suitable host for expression of recombinant proteins from baculovirus expression systems3,4 (e.g. Invitrogen’s Bac-to-Bac® and Bac-N-Blue™ Expression Systems). The Sf9 cell line exhibits the following general features. Insect Cell Culture Products A variety of insect cell lines and Gibco™ cell culture products are available from Invitrogen to facilitate baculovirus-mediated expression of your recombinant protein in insect cells. For more information about the insect cell lines and Gibco™ cell culture products, see our Web site (www.doorway.ru) or contact Technical.